〔 摘要〕　 目的 　 研究早期干预对缺氧缺血性脑损伤 ( HIBD) 大鼠学习记忆功能的影响及其作用机制。方法 　选用
SD大鼠建立宫内 HIBD动物模型 , 随机分为非干预组和干预组 , 非干预组与正常对照组常规饲养 , 对干预组采取早期触摸
和丰富环境刺激。干预 28d后 , 通过三等臂 Y型迷宫试验检测各组大鼠的学习记忆功能 , 而后取大鼠额皮质和海马组织进
行病理观察 , 并采用DNA 缺口原位末端标记法 ( TUNEL 反应法) 检测凋亡细胞 , 观察脑组织神经元凋亡情况。结果 　单
纯 HIBD组大鼠学习获得与记忆保持能力明显低于正常对照组 ( P < 0101) , 但 HIBD 干预组 Y迷宫测试成绩则优于 HIBD
非干预组 ( P < 0101) 。同时 , HIBD非干预组脑额皮质和海马 CA1 区神经元缺失远较正常组多 ( P < 0101) , 而 HIBD 干预
组与 HIBD 非干预组之间神经元数量的差异则不那么显著。但 HIBD 干预组脑额皮质和海马神经元凋亡百分率明显低于
HIBD非干预组 ( P < 0101) 。结论 　早期干预可减轻缺氧缺血性损伤脑组织神经细胞凋亡 , 该作用可能是早期干预促进
HIBD大鼠脑功能修复的机制之一。
〔 关键词〕　 缺氧缺血性脑损伤; 　 早期干预; 　 大鼠; 　 学习记忆; 　 神经元; 　 凋亡
〔 中图分类号〕　R74311 　　〔 文献标识码〕　A
CHANGES OF APOPTOTIC NEURONS IN THE BRAIN OF NEONATAL RATS AFTER
HYPOXIA2ISCHEMIA AND EARLY INTERVENTION
Chen Min , Chen Minrong1
, Chen Yuanhui
1
, Chen Daguang1
( Dep artment of Medical L aboratory , Medical Technolog y and Engi neeri n g Col lege ,
Fuj i an Medical Uni versi t y , Fuz hou 350004 , Chi na)
〔 Abstract〕　Objective To explore t he effect of early intervention on f unctional outcome and neuron
apopto sis in t he brain of rat s wit h hypoxic2ischemic brain damage ( HIBD) . Methods SD rat s were used to
establish the model of HIBD. The HIBD rat s were randomly divide into two group s : non2intervention
group and intervention group t hat received t he neonatal handling and was kept in an enriched environ2
ment . Non2intervention group and normal cont rol group were kept in a standard condition. On t he 28t h
days af ter t he operation , the learning2memory ability of rat s in every group was evaluated t hrouth t ri2e2
qual2arm maze test . Terminal deoxynucleotidyl t ransferase2mediated dTUP biotinylated nick end labeling
( TUNEL) met hod was used to detect neuron apopto sis in pref rontal cortex and hippocamp us of rat s. Re2
sult s Compared wit h the normal group , the non2intervention HIBD group was significant ly decreased in t he
Y2maze learning ability ( P < 0101) , while t he learning2memory ability of t he intervention HIBD group
was obviously improved ( P < 0101) . The pat hology differentiation was obvious between the HIBD
group s and the normal cont rol s. The p ref rontal cortex and hippocampal CA1 neurons of t he HIBD group s
were apparently decreased in number as compared with those of t he normal cont rol s ( P < 0101) , while t he
neurons of t he HIBD intervention group were a lit t le more than those of t he HIBD non2intervention group .
At t he same time , t he percentage of TUNEL positive neurons in pref rontal cortex and hippocamp us of t he
intervention group was less t han t hat of t he non2intervention group ( P < 0101) . Conclusion Early interven2
tion can decrease t he percentage of apoptotic neurons in t he brain of rat s wit h HIBD , which may be one of
t he mechanisms of early intervention improving t he f unctional outcome of HIBD rat s.
〔Key words〕　Hypoxic2ischemic brain damage ; 　Early intervention ; 　Rat ; 　Learning2memory ;
　Neuron ; 　Apoptosis